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. 2018 Feb 7;8:2546. doi: 10.1038/s41598-018-20791-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qPCR validation of host lincRNAs in MDMs infected with HIV1 and HIV-2 Day-7 post infection. The data represents results from 3 independent experiments. Values are fold-change plotted on a Log scale for HIV-1 (black) and HIV-2 (red) infected MDMs relative to an uninfected control MDMs. qPCR was normalized with the endogenous GAPDH control and measured the relative amount of target gene. Expression of fold change is calculated as n-fold difference in expression of gene-of-interest relative to GAPDH gene for infected and uninfected cells as n-fold = 2^ − (ΔCt infected −ΔCt uninfected). Each sample was run in triplicate to ensure accurate fold change estimation and the results expressed as mean ± SEM.