Table 3.
Evaluation of extraction methods and sample mass on heroin samples of varying purities.
| Sample | Sample mass (g) | Extraction method | Cycle threshold (Cp) |
|---|---|---|---|
| WP-1 | 1.0 | Maxwell | 32.09 |
| QP | 32.31 | ||
| QS | 30.89 | ||
| 2.0 | Maxwell | 34.38 | |
| QP | 33.72 | ||
| QS | 32.22 | ||
| BPf-1 | 1.0 | QP | 33.10 |
| QS | 29.47 | ||
| 2.0 | QP | 34.2 | |
| QS | 30.49 | ||
| BPc-1 | 1.0 | QP | 27.64 |
| QS | 29.67 | ||
| 2.0 | QP | 28.77 | |
| QS | 30.77 | ||
| NTC | — | — | >35.00 |
| — | — | >35.00 |
Lower crossing point (Cp) values indicate higher DNA concentrations. The no-template controls (NTC) are also included and exhibit Cp values > than 35, which indicates an unreliable signal to accurately determine the presence of significant amplification. Note, the Maxwell RNA LEV method was only used to extract DNA from sample WP-1 because the remaining samples lacked the necessary mass to perform the three extraction methods. QP- Qiagen plant Mini kit, QS- Qiagen Stool Mini Kit.