Table 4.

Primers used for qPCR.

Gene	Accession no. or reference	Forward primer	Reverse primer	Size (bp)	TA (°C)
AREG	NM_214376	GCCATTGCTGCTTTTGTCTCTGCC	TGGCAGTGACCCCGATCTGCT	198	60
BDNF	Solberg et al.54	AGCGTGTGCGACAGCATTAG	GTCCACTGCCGTCTTTTTATCC	60	58
CKM	Seale et al.55	GCAAGCACCCCAAGTTTGA	ACCTGTGCCGCGCTTCT	62	55
EGF	Kennedy et al.56	TCTGAACCCGGACGGATTTG	GACATCGCTCGCGAACGTAG	202	60
EGFR	Kalbe et al.32	TGGAGAAGCTCCCAACCA	CTCTTAATTCCTTGATAGCCACAG	161	60
GATM	NM_001128442	GGCAGCTTGAGATGTTGATCC	TCACCAGTGTTGAGATGAGAGT	256	60
GHR	Rehfeldt et al.57	TGATTCTACCCCCAGTTCCAGTTC	TCAGTCTTTTCATCAGGGTCATCA	187	56
HPRT1	Erkens et al.58	CCGAGGATTTGGAAAAGGT	CTATTTCTGTTCAGTGCTTTGATGT	181	59
IGF1	Kalbe et al.32	CTCTTCGCATCTCTTCTACTTGGC	CCTGTGGGCTTGTTGAAATAAAA	150	60
IGF2	Kalbe et al.32	TGGCATCGTGGAAGAGTG	AGGTGTCATAGCGGAAGAAC	164	57
IGFBP5	Rehfeldt et al.57	GTGTACCTGCCCAACTGTGA	AAGCTGTGGCACTGGAAGTC	158	56
IGF1R	Kalbe et al.32	GATTCAGGCCACCTCTCTCTCC	CCCTCCTACTATCAACAGAACGGC	139	60
MRF4	Maak et al.59	CGCCATCAACTACATCGAGAGGT	ATCACGAGCCCCCTGGAAT	189	60
MSTN	Maak et al.59	CCCGTCAAGACTCCTACAACA	CACATCAATGCTCTGCCAA	141	62
MYF5	Rehfeldt et al.57	CCTGAATGCAACAGCCCT	CGGAGTTGCTGATCCGAT	152	60
MYOD	Rehfeldt et al.57	GGTGACTCAGACGCATCCA	ATAGGTGCCGTCGTAGCAGT	108	60
MYOG	Rehfeldt et al.57	CAACCAGGAGGAGCGAGAC	AGGGTCAGCTGTGAGCAGAT	161	64
MYOT	NM_001099941	GGCTCGCAGATTGCTAGGACCA	GCTGTGGTGAATCTTGTGCGGC	83	60
PAX7	Patruno et al.60	CAACCACATCCGCCACAA	TCTTGGAGACACAGCCATGG	101	58
PRKAA2	Kalbe et al.61	TGACCCCCTGAAACGAGCAACTA	CAATGACAAGATGATAAGCCACTGC	233	55
SLN	NM_001044566	GGCACCCCATAGCACTTCTGACC	GGCAGCCCTTGAGAGCAGCAT	102	60
SORBS1	XM_001924661	CCACTGCAAGCCCTCAGCCTT	GTGACTCTTCGCTGCTGGGCT	106	65

T_A, annealing temperature; AREG - amphiregulin; BDNF – brain derived neurotrophic factor; CKM - creatine kinase, M-type; EGF – epidermal growth factor; EGFR - epidermal growth factor receptor; GATM - glycine amidinotransferase; GHR – growth hormone receptor; HPRT1 - hypoxanthine phosphoribosyltransferase 1; IGF1, 2 – insulin-like growth factor 1, 2; IGFBP5 - insulin growth factor binding protein 5; IGF1R - insulin-like growth factor 1 receptor; MRF4 - muscle-specific regulatory factor 4; MSTN – myostatin; MYF5 – myogenic factor 5; MYOD - myogenic differentiation factor; MYOG – myogenin; MYOT - myotilin; PAX7 – paired box transcription factor 7; PRKAA2 - AMP-activated protein kinase catalytic sub-unit alpha-2; SLN – sarcolipin; SORBS1 – sorbin and SH3 domain containing 1.