Fig. 1.

FGF19 inhibition of hepatic PC production is SHP dependent. a Schematic diagram of the 1C cycle. b From liver ChIP-seq data for mice treated with FGF19²⁶, positions of SHP binding peaks at the promoter regions of key genes involved in the 1C cycle are shown. c, d Effects of FGF19 treatment for 2 h on SHP occupancy determined by ChIP at 1C genes in C57BL/6 mice (c), and comparison of occupancy of SHP at Pemt and Gnmt genes in C57BL/6 and SHP-KO mice (d). e, f C57BL/6 or SHP-KO mice were treated with FGF19 for 6 h, and mRNA levels of 1C cycle genes (e) or 1C cycle metabolites levels (f) were determined by qRT-PCR or LC–MS, respectively. c–f Means ± SD are shown (n = 5 mice), and statistical significance was measured using the two-way ANOVA with the FDR post-test and *P < 0.05, **P < 0.01, NS, not statistically significant