Fig. 2.

FGF15 and SHP are physiological repressors of PC production in the fed state. C57BL/6 mice and SHP-KO mice (a, b) or FGF15-KO and WT littermate (c, d) mice were fasted for 12 h (Fs) or fasted and refed for 6 h (Fd). Hepatic mRNA levels of selected 1C cycle genes (a, c) and 1C cycle metabolites (b, d) were measured by qRT-PCR and LC–MS analysis, respectively. Means ± SD are shown (n = 5 mice), and statistical significance was measured using the two-way ANOVA with the FDR post-test and *P < 0.05, **P < 0.01, NS, not statistically significant