Fig. 7.

Downregulation of Pemt or overexpression of SHP blunts AhR-mediated increases in PC levels and liver steatosis in mice fed HF/HF. a Experimental outline: Mice (n = 5 mice per group) were fed a HF/HF diet for 8 weeks and infected with the indicated adenovirus. For the Ad-CA-AhR injection, Ad-GFP was added so that the same number of viral particles were injected for each group. At 3 weeks after adenoviral injection, livers were collected for further analyses. b Samples from three mice per each group were randomly selected and analyzed by IB (left) and quantification of the bands is shown (right). Full size immunoblots are in Supplementary Figure 12d. c Representative images of livers from the experimental groups. d Oil Red-O staining of frozen liver sections. Scale bar: 100 µM. e Liver TG levels. f mRNA levels of Pemt, Gnmt, and Cyp1a1 as determined by qRT-PCR. g Ratios of SAH/SAM and PC/PE as calculated from levels of these metabolites determined by LC–MS (n = 5). Means ± SD are shown (n = 5 mice), and statistical significance was measured using the b- (right), e–g one-way ANOVA with the FDR post-test. *P < 0.05, **P < 0.01, NS, not statistically significant