Figure 7.

Biphasic effects of GA on chondrocyte adipogenesis. (A) Primary rib chondrocyte cultured with normal medium containing Dex plus GA (50 μM) for 7 d. The oil red O–stained areas are quantified on the right (**p < 0.01; n = 3). (B) GA (50 μM) treatments increased the markers of adipogenesis (**p < 0.01; n = 3). (C) primary rib chondrocyte cultured in the adipogenic medium containing GA (50 μM) over the entire differentiation period (7 d). The oil red O–stained areas and average stained area per cell are quantified on the right (**p < 0.01; n = 3). (D) Primary rib chondrocyte cultured in adipogenesis medium for 5 days with GA added at d 5 for 24 h showed a decrease expression of lipogenesis markers (**p < 0.01; n = 4).