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. 2018 Feb 7;9:533. doi: 10.1038/s41467-018-02894-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Senataxin removes DSB-induced RNA:DNA hybrids in active loci. a Genome browser screenshots representing DRIP-seq reads count before (−4OHT) and after damage induction (+4OHT) at two individual AsiSI sites. Senataxin profiles in both conditions are also shown, together with BLESS enrichment following DSB induction as well as RNA PolII-S2P and RNA-seq prior to DSB induction. b Box plots representing DRIP-seq reads count before (−4OHT) and after (+4OHT) DSB induction at sites displaying AsiSI-induced cleavage (“cut”) or not (“uncut”). Center line: median; box limits: 1st and 3rd quartiles; whiskers: maximum and minimum without outliers. P values are indicated (Wilcoxon–Mann–Whitney test). c Close-up genome browser screenshot of senataxin and RNA:DNA hybrids at an individual DSB upon DSB induction. d Average DRIP-seq (top) and senataxin ChIP-seq (bottom) profiles on a ±5 kb window centered on the 80 AsiSI induced DSBs. e DRIP-qPCR in control (CTRL) and senataxin (SETX#2) depleted cells before (−4OHT) and after (+4OHT) DSB induction in DIvA cells as indicated. The position of the primers used to quantify RNA:DNA hybrids by qPCR are indicated on the genome browser screenshot above. Mean and s.e.m. of three biological replicates is shown