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. 2018 Feb 7;9:533. doi: 10.1038/s41467-018-02894-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Senataxin depletion does not delay repair kinetics. a γH2AX staining performed in untreated or 4OHT-treated AID DIvA cells (4 h), followed by auxin (IAA) addition, after transfection with control or SETX siRNA as indicated. Scale bar: 10 µM. b Quantification of the γH2AX nuclear signal ( > 100 nuclei) from a representative experiment, performed in the above condition. Center line: median; box limits: 1st and 3rd quartiles; whiskers: maximum and minimum without outliers. P values are indicated (unpaired t-test). c Cleavage assay performed in AID-DIvA cells left untreated or treated with 4OHT (4 h) followed by auxin (IAA) addition (30 min, 60 min, and 120 min), after transfection of control or SETX-directed siRNA. Precipitated DNA was analyzed close to two DSBs, found to recruit SETX after 4OHT. The percentage of sites that remain broken for each DSBs after the indicated time of auxin treatment are presented. Average and s.e.m. (n = 3, biological replicates) are shown