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. 2018 Feb 7;8:2561. doi: 10.1038/s41598-018-20951-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characters of Mab-1 antibody. (A) Specificity of MAb-1 against CHO cells (left panel) and A431 cells (right panel). The purple (left) and the green (right) lines in each panel represent the control and Mab-1 groups, respectively. (B) Epitope determination of Mab-1 by ELISA assay. Nine gangliosides were immobilized and followed incubation with purified MAb-1 antibody. (C) Subclass of MAb-1. The method was performed according to the guide of Sino Biological Mouse Mab Antibody isotyping kit.