Fig. 3.

Drosha is required for early 53BP1 localization after DNA damage. a Representative immunofluorescence image of IR-induced 53BP1 foci in A549 cells, 30 mins post 5 Gy IR. Scale bars, 10 µm. b Drosha knockdown significantly impairs 53BP1 foci formation as early as 30 minutes after 5 Gy IR. Violin plots and data points show quantification, as in Fig. 1B, of IR-induced 53BP1 and γH2A.X foci shown in a. 200 cells were counted across 3 biological replicates, ***p ≤ 0.001, Mann-Whitney non-parametric test. c U2OS cells expressing GFP-53BP1 were subjected to laser microirradiation and GFP redistribution was monitored in real time. Images of selected time points post microirradiation are shown. d Quantification of time-course as in c, error bars = SEM, n ≥ 30 cells per condition over 4 replicates