Fig. 6.

No significant enrichment of small RNA is found around 99 known AsiSI cut sites in the genome. Coverage profiles of 21–23 nt non-miRNA small RNAs that map to regions 5 kb on either side of AsiSI cut sites at different time points after induction of damage. Negative coverage refers to those reads mapping to the consensus genome minus strand. Left, 99 cut AsiSI sites (shaded with pale green). Right, same number of random genomic sites with similar transcriptional activity to the 99 AsiSI cut sites, as determined by TPM count and gene length from 4sU-Seq data on control cells (analysis described in Methods). These graphs are centred around a point (denoted as 0) at the same distance from the TSS as the matched AsiSI site. Each time point in the AsiSI transfected samples (red lines) is superimposed on reads from the EV control (black lines). As the EV control has never been exposed to the specific cuts produced by AsiSI, there is no potential for any small RNAs produced by that event to be present. Any reads present in the EV control can therefore be considered background