Fig. 2.
Transcriptomic analyses revealed a common gene signature in type 2 diabetic islets of OD and PPP. (a) Venn diagram showing the number of differentially expressed (DE) genes in T2D OD and PPP islets and in ND OD and PPP islets. The numbers include genes mapping to more than one probe set. Twenty-three differentially expressed genes overlapped between the two datasets (p = 9.1 × 10−12; hypergeometric test assuming a background of 15,165 expressed genes). Heatmaps indicate the number of upregulated (red) and downregulated (blue) genes in OD and PPP islets. (b–f). Box plots for the expression changes of five of the 19 differentially expressed genes that were dysregulated in the same direction in T2D compared to ND in OD and PPP islets. Circles represent outliers. (g) Fold changes in the expression of the 19 commonly dysregulated genes in T2D in islets from IGT PPP (black bars), T3cD PPP (grey bars) and T2D PPP (white bars) vs expression in ND PPP islets. See ESM Tables 7 and 8 for more details. ND, non-diabetic; T2D, type 2 diabetic; T3cD, type 3c diabetic