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. Author manuscript; available in PMC: 2018 Feb 8.
Published in final edited form as: ACS Appl Mater Interfaces. 2018 Jan 24;10(5):4379–4393. doi: 10.1021/acsami.7b14362

Figure 4. Temperature-dependent uptake of αMSH-PEG-Cy5-C′ dots and cellular internalization.

Figure 4

Specific binding and uptake of αMSH-PEG-C dots in M21(a) and B16F10 (b) cells as a function of temperature using 25nM particle concentrations and flow cytometry. Each data point represents the mean ± s.d. of 3 replicates. (c) αMSH-PEG-C′ dot (1 μM, red) colocalization with a lysosomal marker (LAMP1-GFP) after 4h incubation using M21 cells. Colocalized vesicles (yellow); Scale bar: 10 μm.