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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Int J Biochem Cell Biol. 2017 Dec 20;95:73–84. doi: 10.1016/j.biocel.2017.12.014

Fig 5. ACTN4Y11/13E decreases its binding activity to actin.

Fig 5

(A) WT and mutant ACTN4 purified from bacteria were incubated with actin in the presence of ATP at room temperature for 1h followed by ultracentrifugation and SDS-gel electrophoresis. After staining with coomassie blue G250 and destaining, the densities of ACTN4 protein bands were quantified using Image J software. S and P stand for soluble and insoluble (in pellet) ACTN4 protein, respectively. (B) Saturation binding curves were generated by nonlinear regression from quantified results of coomassie-blue stained polyacrylamide gels.