Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
WT and mutant ACTN4 purified from bacteria were incubated with recombinant m-calpain in the presence of ATP at 30°C for 1h followed by termination and SDS-gel electrophoresis. Gels were stained with coomassie blue G250 and completely destained. Representative images present the intact and cleaved ACTN4 by indicated concentration of m-calpain for (A), (B) and (C).
