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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Int J Biochem Cell Biol. 2017 Dec 20;95:73–84. doi: 10.1016/j.biocel.2017.12.014

Fig 7. ACTN4Y11/13E enhances melanoma cell invasion.

Fig 7

(A) Melanoma cell line WM983b stably expressing WT ACTN4-eGFP or ACTN4Y11/13E-eGFP was cultured on collagen I (1 μg/ml) coated coverslips overnight. After fixation, images were taken from random chosen area and representative cells are shown. The ratio of cell length to width was analyzed using Image I software. N=50 cells. (B) Migration and (C) Invasion of 983b cells expressing either WT ACNT4-eGPF or ACTN4Y11/13E-eGFP. (D) Melanoma WM1158 ACTN4 knockdowned cell line expressing WT ACTN4-eGFP or ACTN4Y11/13E-eGFP was cultured on collagen I (1 μg/ml) coated coverslips overnight. Images were taken from random chosen area. (E) Invasion of WM1158 ACTN4 knockdowned cell line expressing WT ACTN4-eGFP or ACTN4Y11/13E-eGFP. Data are mean ± SD of three independent experiments. Statistical analysis was performed using Student’s t-test. * P < 0.05.