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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2017 Nov 9;134:7–15. doi: 10.1016/j.prostaglandins.2017.11.001

Table 1.

PG MRM mass transitions and average concentration across all patients.

Standard RT (min) MRM mass transition m/z Average estimated PG concentration
PGA1 13.5 335/273 ND
PGA2 13.3 333/189 ND
PGD2 12.6 351/189 ND
PGE1 12.4 353/317 70 ± 21 pg/ml
PGE2 12.3 351/189 638 ± 235 pg/ml
6-keto-PGF1α 10.7 369/245 ND
PGF1α 11.9 355/311 38 ± 8 pg/ml
PGF2α 11.9 353/193 654 ± 269 pg/ml*
8-iso-PGF2α 11.5 353/193 50 ± 6 pg/ml*
PGF3α 11.3 351/191 ND

ND, not determined because most HFF samples had low or undetectable PG concentration. Mean ± standard error of the mean (SEM) is shown. Average estimated concentration is in picograms (pg) per milliliter (ml) of HFF, assuming a 50% extraction/detection efficiency (see Methods). HFF was concentrated 10-fold in 80% MeOH prior to analysis. RT, Retention Time in minutes.

*

this peak may contain multiple 15-F2-isoprostanes (see Discussion).