Skip to main content
. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Neurobiol Dis. 2017 Dec 21;111:127–137. doi: 10.1016/j.nbd.2017.12.009

Figure 6. Estrous cycle-linked changes in the synaptic AMPAR-mediated transmission and GluA1 and GluA2 subunit expression.

Figure 6

A: Traces from representative animals in estrus and diestrus phases illustrating mEPSCs recorded from CA1 pyramidal neurons. B: Averaged mEPSC from an animal in estrus (red) and diestrus (blue) stages. C: Distribution of mEPSC amplitudes recorded from the cell shown in C. The inset shows the mean of the median mEPSC amplitude (n= 16 CA1 neurons from 8 animals in estrus and 10 neurons from 6 animals in diesturs, *p<0.05, Student’s t-test. The frequency of mEPSCs (0.89 ± 0.26 Hz vs 0.74 ± 0.13 Hz), 10–90% rise-time (4.56 ± 0.31 ms vs 3.81 ± 0.27 ms) and weighted decay (11.29 ± 0.72 ms vs 11.56 ± 1.07 ms) were not different between animals in estrus and diestrus stages. D: Expression of GluA1 and GluA2 in the hippocampal proteins isolated from animals in the four stages of estrous cycle. E: Subunit expression in animals in various stages of estrous cycle normalized to that of β-actin (n= 7, * p<0.05 vs diestrus, Friedman’s test followed by Dunn’s multiple comparison test). F: GluA1 and GluA2 subunit expression in RU-486 (30 mg/kg, daily) or vehicle-treated animals. The treatment was started when animals were in diestrus stage and continued until the following estrus stage. G: The subunit expression normalized to that of β-actin (n= 6 for GluA1 and 7 for GluA2, * p<0.05, paired t-test).