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. Author manuscript; available in PMC: 2018 Jul 15.
Published in final edited form as: Nat Med. 2018 Jan 15;24(2):194–202. doi: 10.1038/nm.4464

Extended Data Fig. 6. Effect of glutamine withdrawal (A/B) or combinatorial ASCT2 substrate withdrawal (C/D) on cellular viability and cell death in V-9302 sensitive human CRC cell lines.

Extended Data Fig. 6

Cell lines propagated for 48 hrs in either glutamine-depleted or ASCT2-substrate-depleted media (without alanine, serine, cysteine, threonine, glutamine, asparagine, methionine, glycine, leucine, valine, glutamate); Multi-Tox Glo assay. (A) Cell viability and (B) cell death with glutamine withdrawal (C) Cell viability and (D) cell death with ASCT2-substrate withdrawal. Percent change relative to standard media control. n.s. = not statistically significant; ** = p < 0.01; **** = p < 0.0001 as determined by Student’s t test. Error bars represent ± std. dev.