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. Author manuscript; available in PMC: 2019 Feb 6.
Published in final edited form as: Structure. 2018 Jan 18;26(2):270–281.e4. doi: 10.1016/j.str.2017.12.012

Figure 3. Catalytic activity of the LET-23 kinase domain in solution and associated with lipid vesicles.

Figure 3

(A) Specific activity of the LET-23 and human EGFR kinase domain, each at 1 μM, in solution (light grey) and linked to vesicles (dark grey) containing 5 mole percent DOGS-NTA-Ni. (B) Concentration-dependent activation of the LET-23 kinase domain upon association with lipid vesicles. The specific activities of the kinase domain in solution or on vesicles containing increasing mole percentages of DOGS-NTA-Ni are shown. In all measurements, the ratio of protein to total lipids in bulk solution was fixed at 1 μM and 12.5 μM, respectively. Data are represented as mean ± S.D. (C) Alignment of the activation loop sequences of LET-23 and the human EGFR kinase domain.