Table 1.
MIC valuesa (in μg/mL) (the corresponding values in μM are provided in Table S1) determined for compounds 5-16 and for four control antifungal agents (AmB, CAS, FLC, and VOR) against various yeast strains and filamentous fungi. Each experiment was performed in triplicate.
| Yeast strains | Filamentous fungi | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cpd# | A | B | C | D | E | F | G | H | I | J | K | L | M |
| 5 | 7.8 | >31.3 | 15.6 | 31.3 | 31.3 | >31.3 | 31.3 | 15.6 | 7.8 | 0.975 | >31.3 | >31.3 | >31.3 |
| 6 | 0.975 | >31.3 | 31.3 | 7.8 | 31.3 | 15.6 | 31.3 | 7.8 | 0.975 | 0.06 | >31.3 | 1.95 | 15.6 |
| 7 | 0.48 | 15.6 | 3.9 | 3.9 | 7.8 | 7.8 | 7.8 | 0.975 | 0.48 | 0.03 | 7.8 | 0.975 | 3.9 |
| 8 | 0.975 | 15.6 | 3.9 | 1.95 | 15.6 | 7.8 | 15.6 | 0.975 | 0.975 | 0.48 | 7.8 | 1.95 | 3.9 |
| 9 | 1.95 | >31.3 | >31.3 | 7.8 | 31.3 | >31.3 | 31.3 | 0.975 | 3.9 | 0.48 | >31.3 | 3.9 | 7.8 |
| 10 | >31.3 | >31.3 | 31.3 | 31.3 | 3.9-31.3 | >31.3 | >31.3 | 0.975 | 3.9 | 0.48 | >31.3 | 3.9 | >31.3 |
| 11 | >31.3 | >31.3 | 31.3 | 31.3 | >31.3 | >31.3 | >31.3 | >31.3 | >31.3 | >31.3 | >31.3 | >31.3 | >31.3 |
| 12 | 3.9 | >31.3 | 31.3 | 3.9 | 15.6 | 3.9 | 7.8 | 15.6 | 1.95 | 0.24 | >31.3 | 7.8 | 31.3 |
| 13 | >31.3 | >31.3 | >31.3 | >31.3 | >31.3 | >31.3 | >31.3 | >31.3 | >31.3 | 31.3 | >31.3 | >31.3 | >31.3 |
| 14 | 31.3 | >31.3 | 31.3 | 31.3 | >31.3 | 31.3 | >31.3 | 31.3 | 31.3 | 0.975 | >31.3 | 15.6 | >31.3 |
| 15 | 31.3 | 31.3 | >31.3 | 31.3 | 3.9-31.3 | 31.3 | >31.3 | 31.3 | 31.3 | 3.9 | 31.3 | >31.3 | >31.3 |
| 16 | >31.3 | >31.3 | >31.3 | 31.3 | 31.3 | 31.3 | 31.3 | 7.8 | >31.3 | 7.8 | 31.3 | 7.8 | 15.6 |
| AmB | 3.9 | 3.9 | 1.95 | 0.975 | 1.95 | 3.9 | 3.9 | 1.95 | 3.9 | 1.95 | 15.6 | 3.9 | 3.9 |
| CAS | 0.975 | 0.24 | 0.06 | 0.12 | 0.12 | 0.24 | 0.48 | 0.06 | 0.48 | 1.95 | >31.3 | >31.3 | >31.3 |
| FLC | 62.5 | >125 | 15.6 | >125 | >125 | 62.5 | 62.5 | >31.3 | >31.3 | 1.95 | 62.5 | 62.5 | 62.5 |
| VOR | 0.24 | 3.9 | 1.95 | 1.95 | 0.975 | 7.8 | 1.95 | 0.06 | 0.12 | <0.03 | 0.24 | 0.12 | 0.12 |
Yeast strains: A = Candida albicans ATCC 10231, B = C. albicans ATCC 64124, C = C. albicans ATCC MYA-2876(S), D = C. albicans ATCC 90819(R), E = C. albicans ATCC MYA-2310(S), F = C. albicans ATCC MYA-1237(R), G = C. albicans ATCC MYA-1003(R), H = Candida glabrata ATCC 2001, I = Candida krusei ATCC 6258, J = Candida parapsilosis ATCC 22019. NOTE: Here, the (S) and (R) indicate that ATCC reports these strains to be susceptible (S) and resistant (R) to ITC and FLC.
Filamentous fungi: K = Aspergillus flavus ATCC MYA-3631, L = Aspergillus nidulans ATCC 38163, M = Aspergillus terreus ATCC MYA-3633.
Known antifungal agents: AmB = amphotericin B, CAS = caspofungin, FLC = fluconazole, VOR = voriconazole.
For yeast strains: MIC-0 values are reported for FLC analogues 5-16, AmB, and CAS, whereas MIC-2 values are reported for azoles. For filamentous fungi, MIC-0 values are reported for all compounds.