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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Fungal Genet Biol. 2018 Jan 5;111:1–6. doi: 10.1016/j.fgb.2018.01.003

Fig. 1.

Fig. 1

Gene deletion strategy. A. Transformation with a linear molecule containing the Aspergillus terreus pyrG gene (AtpyrG) flanked by left and right flanking sequences from the target gene to be deleted results in replacement of the target gene by AtpyrG. B. Transformation with a linear molecule containing only the left and right flanking sequences from the target gene results in the removal of the AtpyrG gene. Transformants in which AtpyrG has been removed can be selected on 5-fluoroorotic acid which selects against strains carrying a functional pyrG gene. The result is a deletion of the target gene with no selectable marker sequences left behind. The AtpyrG gene can be reused in subsequent transformations.