Fig. 4.

Complementation of newly created deletions with homologous A. terreus genes. The plates in the top row contain minimal medium fully supplemented for all seven nutritional requirements of LO9771. LO9771 grows as well as the parental strain LO4389. The bottom strain on each plate is LO9771 transformed with a fragment carrying an A. terreus gene homologous to one of the genes deleted in LO9771. The bottom row of plates contain minimal medium lacking a supplement required due to one of the newly created deletions. Thus, in the minus choline plate, LO9771 does not grow because of the choline requirement created by the choA deletion. However, the white strain at the bottom of the plate is LO9771 that has been transformed with the AtchoA gene (inserted at the wA locus), and it grows as well as the parental strains. Similarly, the AtlysB, AtpabaA, and AtbiA genes fully complement the corresponding deletions.