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. Author manuscript; available in PMC: 2019 Feb 6.
Published in final edited form as: Structure. 2018 Jan 11;26(2):187–198.e4. doi: 10.1016/j.str.2017.12.010

Figure 6. FXIaiPD prevents inhibition of FXIa catalytic activity by DEF and related anti-FXIa mAbs.

Figure 6

(A) Initial reaction rates from a FXIa-mediated SN-59 hydrolysis assay in presence of increasing concentrations of FXIaiPD. FXIa and FXIaiPD were mixed before adding 10 nM DEF IgG, followed by SN-59 peptide to start the reaction. Data shown are representative of two independent experiments with similar results.

(B) Initial reaction rates plotted for FXIa assay in which FXIaiPD was mixed with the indicated concentration of DEF or related anti-FXIa mAbs of different potency, all at a in a 10 to 1 molar ratio, prior to addition of FXIa and SN-59 peptide to start reaction. Data shown are mean of duplicate determinations. This experiment was repeated two times with similar results.

(C) Initial reaction rates plotted for FXIa assay, where decoy is pre-mixed in at varying ratios with the small molecule FXIa active site inhibitor SM1 (at 500 and 200 nM), prior to addition of FXIa and SN-59 peptide to start the reaction. Data shown are mean +/− SEM (n= 3).