Table 1.

Primer, Probe, and IC Sequences

Test no.	Primer/probe	Sequence	Label	Concentration (nmol/L)
1	Forward primer	5′-CTAGGCTGCACGCGTGCTACACTGATA-3′	None	40
	Reverse primer	5′-TAATTGCAATAATCTATCCCCATCACG-3′	5′ Biotin	120
	Probe for all 5 Plasmodium species	5′-GAAAAGTGTAGGTAATCTTTATCA-3′	3′ DIG	50
	Probe for P. falciparum	5′-ATATTTGTATCTTTGCTTATATT-3′	3′ FAM	30
	Probe for IC	5′-CCGCTATTTCATAGTAAGTC-3′	3′ DNP	30
	IC 1	5′-CTAGGCTGCACGCGTGCTACACTGATACAACATTGACAACTAAAACCGCTATTTCATAGTAAGTCCTTCGTCTATGACTATCTGTGCCAAATCATCTAGCAAACTGCTAATCCAATTTATTTCATCAAATCGTGATGGGGATAGATTATTGCAATTA-3′	None	500 Copies
2	Forward primer	5′-CGATTCAGCTTGCTGTTTCGTA-3′	None	50
	Reverse primer	Same as in test 1	None	100
	Probe for P. vivax	Same as in test 1	3′ DIG	30
	Probe for IC	Same as in test 1	3′ DNP	30
	IC 2	5′-CGATTCAGCTTGCTGTTTCGTATTTTTCCTCAACATTGACAACTAAAACCGCTATTTCATAGTAAGTCCTTCGTCTATGACTATCTGTGCCAAATCATCTAGCAAACTGCTAATCCAATTTATTTCATCAAATCGTGATGGGGATAGATTATTGCAATTA-3′	None	2000 Copies

Forward and reverse primer-binding regions are underlined.