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Fragmentation spectra of the G2F glycoform of IgG1 obtained under the following conditions: (A) optimal rolling CE based on the charge state and precursor mass of the peptide and (B) optimal rolling CE for maximum recovery of the Y-ion representing loss of one arm. The yield of the “soft” fragment m/z 1297.0 is, in this case, approximately 45% of the precursor ion intensity.
