Figure 5.

Hormone receptor expression of cells within the recellularized scaffolds after 28-day hormone treatment. Decellularized scaffold were seeded with primary cells and allowed to recellularize for 2 weeks before hormone treatment. At the end of the experiment, IHC staining was also used to determine the expression of estrogen receptor (A) and progesterone receptor (B) in the different treatment groups E2 (top row), E2+P4 (middle row), and E2+P4+dbcAMP (bottom row). Representative images from recellularized scaffolds using cells from patients in the proliferative phase are shown (n = 3). One recellularized scaffold per patient was used in each treatment group. Scale bars represent 20 μm.