Major histocompatibility complex class I expression on NLRP2 clones upon IFNγ or TNFα stimulation. (A) Graph depicts mean fluorescence intensity (MFI) of HLA-C (mAb clone DT9) protein expression on JEG3, wild-type (WT) heterozygote (HZ), and knockout (KO) clones in the absence of stimulation or stimulated with IFNγ (100 ng/ml) or TNFα (20 ng/ml). Relative protein expression for (B) HLA-C, (C) HLA-E, (D) HLA-G, and (E) W6/32. Relative protein expression levels are plotted as expression on stimulated cells relative to unstimulated cells. Graphs depict median and interquartile range of a least five independent experiments. *P < 0.05 **P < 0.01.