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. 2017 Mar 7;96(4):831–842. doi: 10.1093/biolre/iox009

Figure 5.

Figure 5.

Deletion of NLRP2 increases NF-ƙB p65-P upon TNFα stimulation. (A) Western blot images of NF-κB p65-P and HSP70 protein expression in NLRP2 wild-type (WT) and knockout (KO) JEG3 clones stimulated with 20 ng/ml TNFα for 0, 5, 15, 30, and 45 min. (B) Western blot images of HSP70, NF-κB p65, and IκBα expression in NLRP2 wild-type (WT, left) and knockout (KO, right) JEG3 clones stimulated with 20 ng/ml TNFα for 0, 5, 15, and 30 min. (C) Western blot quantification of NF-κB p65-P, NF-κB p65, and IκBα relative to HSP70 expression in NLRP2 wild-type (gray bars) and knockout (black bars) JEG3 clones stimulated with 20 ng/ml TNFα for 0, 5, 15, 30, and 45 min of one representative sample. (D) Graphs depict the fold change (FC) of NF-κB p65-P, NF-κB p65, and IκBα protein in KO clones relative to WT clones.