NLRP2 knockdown in primary EVT increases phosphorylation of NF-κB p65. (A) Western blots of NF-κB p65-P, NLRP2, HSP70, and HLA-G protein expression in primary EVT transduced with scrambled (SC) and NLRP2 (KD) shRNA stimulated without or with 20 ng/ml TNFα for 15 min. Graphs depict (B) NF-κB p65-P, (C) NLRP2, and (D) HLA-G relative to HSP70 in two primary EVT (EVT1 and EVT2) isolates transduced with scrambled (SC) and NLRP2 (KD) shRNA. NF-κB p65-P is depicted without or with 20 ng/ml TNFα for 15 min.