Figure 6.

Effect of DMSO and quercetin on the protein tyrosine phosphorylation of cold-stored sperm during capacitation. Cauda epididymides were taken from sacrificed male mice and stored in Lifor, Lifor-DMSO, or Lifor-DMSO-Q at 4°C for 7 days. After storage, sperm were isolated and incubated in BSA-free Toyoda Yokoyama Hoshi medium containing 0.75 mM MBCD and 1.0 mg/mL polyvinyl alcohol. The protein tyrosine phosphorylation of cold-stored sperm was assessed by western blot analysis. α-Tubulin was used as the loading control. (A) Sperm stored in Lifor were incubated for 0 or 60 min. Fresh sperm was used as the control. (B) Sperm stored in Lifor, Lifor-DMSO, or Lifor-DMSO-Q were incubated for 60 min. DMSO, dimethyl sulfoxide. Q, quercetin.