Table 1.
Gene-specific primer sequences used in realtime PCR and/or in situ hybridization.
| PCR product | ||
|---|---|---|
| Gene | Primer sequence | size (bp) |
| Atp6v0a1 | F: AGGCAGCTGCTAAGAACA | 395 |
| R: AAAATCTCCGAACATCACAG | ||
| Atp6v0a2 | F: CATCTACCACATGCTCAACA | 388 |
| R: AGTCTGGGGTGATTCTCATT | ||
| Atp6v0a3 | F: GCTACTGCTGGAGACCTTG | 380 |
| R: CTGTTCTCCAAGGTGGATG | ||
| Atp6v0a4 | F: AAACAGAGTCTCACCGACAG | 391 |
| R: CCAACCTGTTCTGTGGAGT | ||
| Atp6v0b | F: TACACAGTCACCATCAGCAG | 383 |
| R: GAAGGGGAAGGTGATGATAG | ||
| Atp6v0c | F: TGGTGGTGGCAGTACTTATC | 398 |
| R: GCACTAGGACACTGCACATT | ||
| Atp6v0d1 | F: TGAAGCTGC ACCTACAGAGT | 390 |
| R: TTCATCTCATCAAGGTCCTG | ||
| Atp6v0d2 | F: GAGATGGAAGCTGTCAACAT | 398 |
| R: TCTGCCACTCTCTTCATCTG | ||
| Atp6v0e1 | F: GGACCACAGTTGAAAAATGA | 384 |
| R: GTCTCGCAGCAATTCTTAAA | ||
| Atp6v0e2 | F: CACCCATCTGTATGACCATC | 390 |
| R: TATTTCAACACGGTGGAGAC | ||
| Mcoln1 | F: CCTCTTCATCTACATGGTGC | 241 |
| R: CTTGGATCAGTTCACCAGC | ||
| Tpcn1 | F: ATGCCTCTGATGGAACAGG | 368 |
| R: ACCTTCCTGGAGGTAGATTG | ||
| Clcn7 | F: CATGGCCAACGTCTCTAAG | 341 |
| R: GGTTGATTCGTCTTTCCTCC | ||
| Cftr | F: CGAGCCAAAAGCATTGACC | 307 |
| R: GTGGATAAGCTGGGGATTCT | ||
| Hprt1 | F: GCTGACCTGCTGGATTACAT | 172 |
| R: CAATCAAGACATTCTTTCCAGT | ||
| Gapdh | F: GCCGAGAATGGGAAGCTTGTCAT | 205 |
| R: GTGGTTCACACCCATCACAAACAT | ||
| Abp1 | F: TACCCTAATGGTGTGATGGA | 398 |
| R: TCAGCCATAGAGTGGATCTG |
F: forward primer; R: reverse primer, 5΄→3΄.