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. 2017 Feb 20;96(3):707–719. doi: 10.1095/biolreprod.116.142828

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© The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — EGFP immunohistochemistry of various tissues from F3 offspring. Paraffin embedded tissue sections were stained with anti-EGFP antibody. Only germ cells in DDX4-EGFP testes (A) and ovaries (C) were EGFP positive. No staining was evident in wildtype testis (B) and ovary (D) sections, or any of the other organs that were evaluated (E–L). Sections were not counterstained with DAPI to allow observation of the different levels of autofluorescence in intestine, spleen, lung, and kidney tissue. Scale bars = 10 μm.