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. 2017 Feb 20;96(3):707–719. doi: 10.1095/biolreprod.116.142828

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© The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — EGFP is expressed in germ cells throughout germ lineage development and spermatogenesis in postnatal transgenic DDX4-EGFP testes. Germ cells are identified by positive DDX4 staining at 1, 8, 14, and 28 dpp, and in adult testes (left column top to bottom). EGFP was consistently expressed in all DDX4-positive germ cells during postnatal development and during spermatogenesis (center column top to bottom), as confirmed in merged images (right column top to bottom). Scale bar = 50 μm.