Fig. 6.

PHGR1 is a potential marker of lymph node metastasis. a Relative PHGR1 mRNA levels in colon cancers (CC, n = 10), pancreatic cancers (PaC, n = 5), breast cancers (BC, n = 10), prostate cancers (PC, n = 10), non-small cell lung cancers (LC, n = 10), normal lymph nodes (nLN, n = 10), and lymph nodes with known colon cancer metastases (mLN, n = 10). b Relative KRT20 mRNA levels in the same tissue samples as in (a). c Relative PHGR1 mRNA levels in mucosa (n = 76) and tumor (n = 209) tissue samples from patients with operable colon cancer. d Relative PHGR1 and KRT20 mRNA levels in the same samples as in (c). e Immunohistochemical staining of PHGR1 protein in CRC lymph node metastases. Tissue sections from four different lymph nodes with known metastases were stained with PHGR1 antibody (brown) and nuclear counterstain (blue). The images were obtained with a 2.5× microscope objective