Table 2.
Estimates of DNA sequence polymorphism and tests of neutrality at PfAMA-1 among global Plasmodium falciparum
| Isolates | Segregating sites (S) | Singleton variable sites | Parsimony informative sites | Total no. of mutations | K | H | Hd ± SD | π ± SD | dN/dS | Tajima’s D | Fu and Li’s D | Fu and Li’s F |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Myanmar (n = 58) |
78 | 16 | 62 | 79 | 19.886 | 37 | 0.948 ± 0.019 | 0.01064 ± 0.00053 | 6.524 | 0.57189 (P > 0.1) |
0.16773 (P > 0.1) |
0.38506 (P > 0.1) |
| Thailand (n = 80) |
58 | 5 | 53 | 61 | 20.621 | 21 | 0.920 ± 0.013 | 0.01103 ± 0.00020 | 6.698 | 2.22215 (P < 0.05) |
0.95723 (P > 0.1) |
1.75160 (P < 0.05) |
| Philippines (n = 55) |
61 | 3 | 58 | 62 | 21.232 | 19 | 0.916 ± 0.019 | 0.01136 ± 0.00027 | 7.200 | 1.95452 (0.1 > P > 0.05) |
1.61226 (P < 0.05) |
2.07959 (P < 0.02) |
| PNG (n = 90) |
68 | 2 | 66 | 75 | 22.923 | 28 | 0.954 ± 0.007 | 0.01226 ± 0.00016 | 7.497 | 1.80824 (0.1 > P > 0.05) |
1.67585 (P < 0.05) |
2.07602 (P < 0.02) |
| Solomon Islands (n = 50) |
56 | 3 | 53 | 57 | 22.044 | 9 | 0.862 ± 0.021 | 0.01179 ± 0.00027 | 7.023 | 2.54552 (P < 0.05) |
1.54470 (P < 0.05) |
2.28042 (P < 0.02) |
| Vanuatu (n = 85) |
50 | 6 | 44 | 50 | 18.905 | 5 | 0.633 ± 0.028 | 0.01012 ± 0.00031 | 6.471 | 2.89729 (P < 0.01) |
0.86419 (P > 0.1) |
2.00393 (P < 0.05) |
| Ghana (n = 37) |
75 | 7 | 68 | 85 | 26.261 | 32 | 0.992 ± 0.008 | 0.01405 ± 0.00034 | 7.423 | 1.06738 (P > 0.1) |
1.21791 (P > 0.1) |
1.38496 (P > 0.1) |
| Tanzania (n = 62) |
81 | 8 | 73 | 89 | 25.444 | 47 | 0.989 ± 0.005 | 0.01361 ± 0.00021 | 8.135 | 1.18188 (P > 0.1) |
1.16354 (P > 0.1) |
1.40203 (P > 0.1) |
K, average number of pairwise nucleotide differences; H, number of haplotypes; Hd, haplotype diversity; π, observed average pairwise nucleotide diversity; dN, rate of non-synonymous mutations; dS, rate of synonymous mutations