Fig. 1.

FL3 inhibits the growth and proliferation of UCB cell lines. a The CCK-8 assay showed that of the flavaglines tested, FL3 most potently inhibited the cell viability of UCB T24 cells. b After incubation with indicated concentrations of FL3 or paclitaxel (positive control) in 5637, T24, and BIU cells for 24 h or 48 h, absorbance of the treated cells was measured at 450 nm. Cell viability was expressed as the percentage of absorbance of cells treated with FL3 or paclitaxel compared with control. c CCK-8 assay was performed to examine the cytotoxicity of FL3 and paclitaxel (positive control) to normal bladder uroepithelial SV-HUC-1 cells. d Cell colony formation experiments were performed in T24 and BIU cell lines to measure the effects of FL3 on cell proliferation. Histograms display the mean number of colonies, and the number of colonies was shown as the mean ± SD of three independent experiments. *P < 0.05, ***P < 0.001 indicates a significance to control. UCB, urothelial carcinoma of the bladder