Fig. 3.

H19 suppressed miR-148b expression by direct interaction. a The putative binding sites between H19 and miR-148b and the mutant sites in H19-MUT reporter were displayed. b The expression patterns of GAPDH, U6 and H19 in cytoplasm and nucleus of HA-VSMCs were measured. GAPDH acted as a cytoplasm transcript control and U6 served as a nucleus transcript control. The data were displayed as a percentage of GAPDH, U6 and H19 in cytoplasm and nucleus. The total levels were standardized to be 1. c Luciferase activity was detected in HA-VSMCs co-transfected with H19-WT or H19-MUT reporter and miR-148b or its scramble control (miR-con). d RIP and RT-qPCR assays were performed to explore the binding efficiency of H19 and miR-148b to Ago2 protein in HA-VSMCs. e and f HA-VSMCs were transfected with pcDNA3.1 empty vetor, pcDNA-H19 overexpression plasmid, si-con, si-H19#1 or si-H19#2, followed by the detection of H19 e and miR-184 f levels at 48 h after transfection. g Correlation analysis of H19 and miR-148b expressions in 40 cases of AS patient serums. Data are presented as mean ± SEM (n = 3) of at three independent experiments.*P < 0.05