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. 2018 Feb 5;13:791–804. doi: 10.2147/IJN.S152698

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© 2018 Sun et al. This work is published and licensed by Dove Medical Press Limited

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Establishment of OVX model.

Notes: (A) Micro-CT images of distal femoral shafts in Sham and OVX rats, and the morphometric analysis of the bone volume fraction (BV/TV), Tb.N, Tb.Th, and Tb.Sp in femoral shafts between Sham and OVX groups (n=5 per group). (B) Alizarin Red staining for Sham and OVX rats-derived BMSCs after culturing in osteogenic medium for 21 days, and the quantitative analysis of mineralized nodules between Sham and OVX groups at days 14 and 21. Statistical significance is indicated by *p<0.05 compared to Sham group. Magnification: 40×. Scale bar: 200 μm.

Abbreviations: ARS, Alizarin Red S; BMSC, bone marrow stromal stem cells; BV, bone volume; micro-CT, micro-computed tomography; OVX, ovariectomy; Tb.N, trabecular number; Tb.Sp, trabecular separation; Tb.Th, trabecular thickness; TV, tissue volume.