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. 2018 Feb 1;24:643–651. doi: 10.12659/MSM.908237

Figure 2.

Figure 2

Cultured human aortic endothelial cells (HAECs) exposed to hypoxia-reoxygenation and expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) and endothelial nitric oxide synthase (eNOS). (A) Columns indicate the detected optical density (O.D) at 450nm indicating peroxisome proliferator-activated receptor-γ (PPAR-γ) activities in cultured HAECS exposed to hypoxia-reoxygenation for 0, 12, and 24 hours, respectively. (B) The left side shows the captured images of DAF-FM DA fluorescent stain of HAECs. Columns on the right part indicated the fluorescent intensities of DAG-FM DA stain of HAECS exposed to hypoxia-reoxygenation for 0, 12, and 24 hours, respectively. (C) The immunoblots of pPPAR-γ, PPAR-γ, p-eNOS, eNOS and GAPDH in HAECs are shown on the left. Columns on the right show the relative phosphorylation levels of PPAR-γ (white columns) and eNOS (black columns) in cultured HAECS exposed to hypoxia-reoxygenation for 0, 12, and 24 hours, respectively. (D) The immunoblots on the right show the immunoprecipitation analysis of heat shock protein (HSP)90/eNOS interaction in HAECs. Columns on the right indicate the eNOS/HSP90 association (normalized to HSP90) in HAECS exposed to hypoxia-reoxygenation for 0, 12, and 24 hours, respectively. Differences were significant (p<0.05) between a. and b. Differences were significant (p<0.05) between b. and c.