Figure 5.

Cultured human aortic endothelial cells (HAECs) exposed to hypoxia-reoxygenation and analysis of heat shock protein (HSP)90 and endothelial nitric oxide synthase (eNOS). The upper part of this figure demonstrates the immunoprecipitation analysis of HSP90/eNOS in HAECs. Columns on the lower part indicated the eNOS/Hsp90 association (normalized to HSP90) in HAECS exposed to hypoxia-reoxygenation for 0, 12, and 24 hours respectively. Differences were significant (p<0.05) between a. and b. Differences were significant (p<0.05) between b. and c. Differences were significant (p<0.05) between d. and c. Differences were significant (p<0.05) between e. and d. Differences were significant (p<0.05) between f. and e.