Fig. 2. isobolographic analysis of the effect of benznidazole (Bnz) 2 (A), 3 (B) and 4 (C) and their combinations on epimastigotes. Dotted lines correspond to the predicted positions of the experimental points for additive effects and continuous lines correspond to the experimental findings. Points below the dotted line indicate a synergistic effect; points above the dotted line indicate an antagonistic effect. The experiments were repeated as it is indicated in Material and Methods section. The points show median values.