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. 2017 Dec 31;2017:2904150. doi: 10.1155/2017/2904150

Figure 8.

Figure 8

Effects of UPARANT on gliosis and retinal cell death. (a, c) Transcript levels of GFAP (a) and caspase-3 (c) were evaluated by qPCR. Data were analyzed by the formula 2−∆∆CT using Rpl13a and Hprt as internal standards. (b, d) Protein levels of GFAP (b) and active caspase-3 (d) were evaluated by Western blot and densitometric analysis using β-actin as the loading control. In untreated or vehicle-treated SDT rats, levels of GFAP and caspase-3 were increased with respect to SD rats, while UPARANT treatment reduced this increase. ∗P < 0.01 and ∗∗P < 0.001 versus control; §P < 0.05, §§P < 0.01, and §§§P < 0.001 versus vehicle (one-way ANOVA followed by Newman–Keuls' multiple comparison posttest; power values: 0.94 (a), 0.99 (b), 0.87 (c), and 0.99 (d)). Each column represents the mean ± SEM of data from 3 independent samples, each containing 1 retina.