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. Author manuscript; available in PMC: 2018 Feb 10.
Published in final edited form as: J Control Release. 2016 Dec 24;247:1–18. doi: 10.1016/j.jconrel.2016.12.021

Figure 3. MkMPs are quickly endocytosed by HSPCs.

Figure 3

MkMPs were stained with CFDA-SE (green) dye and then cocultured for 3–5 hours with HSPCs from d3 of CD34+ -cell Mk cultures. Fluorescent and Differential Interference Contrast (DIC) images were collected via confocal microscopy. (A) Vehicle control cultures without fluorescent MkMPs display no background green signal. (B–C) MkMP cocultures (~ 4 hours) contain cells with intact fluorescence particles (MkMPs, red arrow) inside them, diffuse fluorescence staining, or fluorescent MkMPs on their surface. Inserts amplify images to show more details. Scale bar in panels (A–C) represents 20 µm. (D–E) Fluorescent images of two cells from the MkMP cocultures (~4 hours) at different confocal planes with 0.4 µm apart. The numbers on the top left of each image represent (image slice number)/(total number of slices). As the image slice number increases, intact MkMPs indicated by red arrows appear while the other MkMPs indicated by blue arrow disappear, thus demonstrating that these MkMPs are inside the cell.