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. Author manuscript; available in PMC: 2018 Feb 10.
Published in final edited form as: J Control Release. 2016 Dec 24;247:1–18. doi: 10.1016/j.jconrel.2016.12.021

Fig. 4. MkMPs can fuse into HSPC membranes and release their content into the target HSPCs.

Fig. 4

(A–B) MkMPs were stained with CFDA-SE (green) dye and then cocultured with for 3–5 hours with d3 HSPCs from Mk cultures of CD34+ cells. Fluorescent and DIC images were collected via confocal microscopy. (A) Images of cells from the MkMP cocultures demonstrate CFDA-SE dye gradients inside the cells emanating from one or few fluorescent particles (red arrow) on the cell surface; Scale bar, 20 µm. (B) CFDA-SE dye intensity profiles quantitating the dye gradient along the black arrows of cells #1 and #2 in the lower panel (A). (C–D) HSPCs as above were cocultured with MkMPs for 3 and 5 hours and examined using SEM. (C) Representative electron micrographs demonstrate four consecutive stages through which MkMPs (red arrow) were fused into HSPC membranes; scale bar, 1 µm. (D) Percentages of MkMP-HSPC interactions at each stage after 3 and 5 hours of coculture. The data represent the average of two biological replicates ± standard error of mean.