3b.

r_h,apparent plotted as a function of the mole fraction of α-chymotrypsin in cross-gradient mixtures of α-chymotrypsin and bovine trypsin inhibitor at two temperatures. Symbols: measured data at two temperatures, solid curves: best-fits of a model in which the two reactants can reversibly form a 1:1 complex with a temperature-dependent affinity, dashed curve: hypothetical dependence of r_h,apparent upon composition in the absence of heteroassociation. Figure reproduced from [13] by permission.