Table 2:
Mean DNA methylation at imprinted genes and associations with OP exposures
| Genes |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GRB10 | H19 | IGF2 | MEG3 | MEG3-IG | PEG1/MEST | NDN | NNAT | PEG3 | PLAGL1 | SGCE/PEG10 | SNRPN | ||
| Mean % (SD) | 1.82 (0.97) | 88.06 (2.14) | 93.92 (2.14) | 1.78 (2.81) | 80.00 (1.95) | 1.63 (0.52) | 1.52 (1.62) | 1.87 (1.67) | 1.52 (1.41) | 4.08 (5.56) | 3.13 (3.45) | 1.60 (0.94) | |
| Associations with OP exposures | |||||||||||||
| ipPPP | Beta | +0.022 | −0.001 | +0.001 | +0.083* | +0.001 | +0.003 | +0.074 | +0.065 | +0.051 | +0.137 | +0.09 | +0.068* |
| (P-value) | (0.24) | (0.66) | (0.78) | (0.0005) | (0.82) | (0.92) | (0.03) | (0.06) | (0.13) | (0.19) | (0.06) | (0.0039) | |
| OR | 1.26 | 0.71 | 0.76 | 2.59 | 1.54 | 0.89 | 2.73 | 1.81 | 1.53 | 1.49 | 1.68 | 2.52 | |
| (95%CI) | (0.44; 3.60) | (0.24; 2.06) | (0.28; 2.10) | (0.94; 7.11) | (0.57; 4.19) | (0.33; 2.42) | (0.93; 7.97) | (0.62; 5.33) | (0.57; 4.09) | (0.54; 4.14) | (0.56; 4.96) | (0.87; 7.28) | |
| DPHP | Beta | +0.006 | −0.003 | −0.001 | +0.064 | −0.004 | +0.020 | +0.008 | +0.051 | +0.034 | +0.261 | +0.104 | +0.066 |
| (P-value) | (0.78) | (0.30) | (0.83) | (0.01) | (0.16) | (0.55) | (0.82) | (0.17) | (0.33) | (0.02) | (0.06) | (0.02) | |
| OR | 1.45 | 0.82 | 1.13 | 2.35 | 0.44 | 0.86 | 1.50 | 1.60 | 1.67 | 3.11 | 1.77 | 5.97 | |
| (95%CI) | (0.46; 4.52) | (0.26; 2.54) | (0.38; 3.32) | (0.78; 7.07) | (0.15; 1.29) | (0.29; 2.55) | (0.49; 4.58) | (0.51; 5.03) | (0.57; 4.89) | (0.96; 10.15) | (0.55; 5.68) | (1.59; 22.48) | |
| BDCIPP | Beta | +0.025 | −0.006 | −0.001 | +0.020 | −0.004 | −0.001 | +0.018 | +0.019 | +0.018 | −0.046 | +0.012 | +0.037 |
| (P-value) | (0.16) | (0.02) | (0.64) | (0.35) | (0.04) | (0.96) | (0.52) | (0.51) | (0.51) | (0.61) | (0.79) | (0.11) | |
| OR | 1.09 | 0.32 | 0.59 | 1.81 | 0.44 | 1.15 | 1.22 | 1.31 | 1.34 | 0.97 | 0.89 | 1.62 | |
| (95%CI) | (0.37; 3.24) | (0.09; 1.09) | (0.20; 1.73) | (0.64; 5.13) | (0.15; 1.27) | (0.40; 3.31) | (0.42; 3.56) | (0.43; 4.02) | (0.48; 3.75) | (0.33; 2.80) | (0.30; 2.63) | (0.55; 4.74) | |
In the upper row the mean methylation % is shown in sperm samples from TIEGER participants. For each OP the associations with DNA methylation at the DMRs were tested by a multivariable robust regression model adjusted for age and BMI status. Beta-coefficients are provided in log-scale. Odds ratios (OR) are shown after a logistic regression analyses on dichotomized normalized values (median used as cutoff), corrected for age and BMI status. Significant results are marked in bold.
The associations that remained significant after correction for multiple testing. OR are shown after a logistic regression analyses on dichotomized normalized values (median used as cutoff), corrected for age and BMI status.