Table 3:
Associations between normalized OPs and DNA methylation at CpG sites of imprinted genes
| OP | DMR_CpG | β-coefficient | Predicted methylation % |
IQ effect size |
P-value | OR (95%CI) | ||
|---|---|---|---|---|---|---|---|---|
| at Q1 | at Q2 | Difference | Relative effect | |||||
| ipPPP | GRB10_CPG6 | 0.021 | 1.896 | 1.982 | +0.087 | +4.6 | 0.0244 | 1.7 (0.6; 4.8) |
| ipPPP | H19_CPG1^ | −0.005 | 95.749 | 95.127 | −0.622 | −14.6 | 0.0438 | 0.4 (0.1; 1.2) |
| ipPPP | MEG3_CPG2 | 0.120 | 1.425 | 1.869 | +0.445 | +31.2 | 0.0003 | 4.7 (1.6; 13.6) |
| ipPPP | MEG3_CPG3 | 0.102 | 0.586 | 0.831 | +0.245 | +41.9 | 0.0036 | 2.4 (0.9; 6.6) |
| ipPPP | MEG3_CPG4 | 0.070 | 1.923 | 2.224 | +0.302 | +15.7 | 0.0008 | 4.5 (1.5; 13.1) |
| ipPPP | MEG3_CPG5 | 0.117 | 0.448 | 0.707 | +0.259 | +57.7 | 0.0021 | 1.7 (0.6; 4.5) |
| ipPPP | MEG3_CPG6 | 0.111 | 0.645 | 0.923 | +0.278 | +43.1 | 0.0291 | 2.2 (0.8; 6.1) |
| ipPPP | MEG3_CPG7 | 0.058 | 1.362 | 1.563 | +0.201 | +14.7 | 0.0111 | 2.7 (1.0; 7.9) |
| ipPPP | MEG3_CPG8 | 0.112 | 1.048 | 1.397 | +0.349 | +33.3 | 0.0060 | 2.4 (0.9; 6.6) |
| ipPPP | NDN_CPG1 | 0.111 | 1.284 | 1.671 | +0.387 | +30.1 | 0.0013 | 4.1 (1.2; 13.4) |
| ipPPP | NDN_CPG4 | 0.097 | 0.953 | 1.239 | +0.286 | +29.9 | 0.0333 | 2.4 (0.8; 6.9) |
| ipPPP | NDN_CPG5 | 0.132 | 1.018 | 1.429 | +0.411 | +40.3 | 0.0134 | 1.9 (0.7; 5.3) |
| ipPPP | NDN_CPG6 | 0.061 | 1.670 | 1.908 | +0.238 | +14.3 | 0.0140 | 2.7 (1.0; 7.5) |
| ipPPP | NNAT_CPG1 | 0.105 | 0.152 | 0.334 | +0.183 | +120.3 | 0.0471 | 4.1 (1.2; 13.4) |
| ipPPP | SGCE_CPG1 | 0.084 | 2.297 | 2.708 | +0.410 | +17.9 | 0.0047 | 3.0 (1.1; 8.4) |
| ipPPP | SGCE_CPG4 | 0.092 | 3.182 | 3.758 | +0.576 | +18.1 | 0.0480 | 2.2 (0.8; 6.3) |
| ipPPP | SGCE_CPG5 | 0.192 | 1.465 | 2.228 | +0.763 | +52.1 | 0.0408 | 1.0 (0.4; 2.9) |
| ipPPP | SNRPN_CPG1 | 0.073 | 2.164 | 2.507 | +0.342 | +15.8 | 0.0072 | 3.0 (1.1; 8.4) |
| ipPPP | SNRPN_CPG2 | 0.088 | 1.269 | 1.569 | +0.300 | +23.6 | 0.0014 | 3.3 (1.1; 9.5) |
| ipPPP | SNRPN_CPG3 | 0.052 | 1.480 | 1.667 | +0.187 | +12.6 | 0.0442 | 3.0 (1.1; 8.3) |
| ipPPP | SNRPN_CPG4 | 0.125 | 0.435 | 0.710 | +0.275 | +63.2 | 0.0104 | 3.1 (1.1; 9.1) |
| DPHP | GRB10_CPG5 | 0.053 | 2.488 | 2.687 | +0.199 | +8.0 | 0.0022 | 2.5 (0.8; 7.9) |
| DPHP | MEG3_CPG1 | 0.082 | 0.087 | 0.185 | +0.098 | +112.1 | 0.0107 | 2.7 (0.8; 8.8) |
| DPHP | MEG3_CPG2 | 0.083 | 1.503 | 1.729 | +0.226 | +15.1 | 0.0337 | 1.3 (0.5; 3.9) |
| DPHP | MEG3_CPG3 | 0.079 | 0.605 | 0.744 | +0.139 | +22.9 | 0.0468 | 2.3 (0.7; 7.0) |
| DPHP | MEG3_CPG4 | 0.053 | 1.975 | 2.143 | +0.168 | +8.5 | 0.0203 | 1.7 (0.6; 5.0) |
| DPHP | MEG3IG_CPG2^ | −0.012 | 79.966 | 78.941 | −1.025 | −6.4 | 0.0061 | 0.9 (0.3; 3.0) |
| DPHP | NNAT_CPG3 | 0.087 | 2.608 | 2.951 | +0.344 | +13.2 | 0.0343 | 1.6 (0.5; 5.0) |
| DPHP | PLAGL1_CPG1 | 0.257 | 1.691 | 2.520 | +0.829 | +49.0 | 0.0338 | 2.4 (0.8; 7.5) |
| DPHP | PLAGL1_CPG2 | 0.264 | 1.559 | 2.374 | +0.815 | +52.3 | 0.0385 | 3.1 (1.0; 10.1) |
| DPHP | PLAGL1_CPG4 | 0.290 | 1.697 | 2.653 | +0.957 | +56.4 | 0.0203 | 3.1 (1.0; 10.1) |
| DPHP | PLAGL1_CPG5 | 0.307 | 1.572 | 2.548 | +0.975 | +62.0 | 0.0045 | 2.8 (0.9; 8.8) |
| DPHP | SGCE_CPG2 | 0.230 | 0.574 | 1.001 | +0.427 | +74.4 | 0.0180 | 1.7 (0.6; 4.9) |
| DPHP | SGCE_CPG6 | 0.121 | 3.388 | 3.979 | +0.591 | +17.4 | 0.0226 | 1.7 (0.5; 5.2) |
| BDCIPP | GRB10_CPG5 | 0.029 | 2.592 | 2.727 | +0.135 | +5.2 | 0.0305 | 1.4 (0.5; 4.2) |
| BDCIPP | H19_CPG2^ | −0.020 | 67.792 | 66.070 | −1.722 | −5.3 | 0.0482 | 0.4 (0.1; 1.3) |
| BDCIPP | H19_CPG3^ | −0.005 | 93.319 | 92.747 | −0.572 | −8.6 | 0.0026 | 0.2 (0.1; 0.8) |
| BDCIPP | MEG3IG_CPG5^ | −0.007 | 80.408 | 79.678 | −0.730 | −3.7 | 0.0021 | 0.3 (0.1; 1.0) |
Multivariable robust linear regression results adjusted for age and BMI status with P-values <0.05; beta-coefficients are provided in log-scale. In bold are the associations that are still significant after D/AP correction, given a nominal α-level of 0.0031. The interquartile (IQ) relative effect (in %) can be derived from the predicted difference in DNA methylation (column 6), comparing OP exposures between Q1 to Q3.
CpG sites that are paternally methylated. At the paternally methylated genes, the IQ effect size represents the relative change in cells that are (aberrantly) unmethylated. At the maternally methylated genes, this value represents the relative change in number of cells that are (aberrantly) methylated. Odds ratios (OR) are shown after a logistic regression analyses on dichotomized normalized values (median used as cutoff), corrected for age and BMI status.