Table 3:
Functional annotation clustering (FAC) as performed in DAVID
| Annotation category | Term | Enrichment score | Gene | FlyBase gene ID | Function | P-value of ANOVA | Significance of ANOVA |
|---|---|---|---|---|---|---|---|
| Interpro | Zinc finger | 10.64 | Bre1 | FBgn0086694 | Ubiquitinates histone H2B on lysine 120 at most RNA Polymerase II transcribed genes | 0.0304 | * |
| pygo | FBgn0043900 | Binds His3 methylated tail to associate with arm as part of Wnt-induced transcription | 0.0030 | ** | |||
| e(y)3 | FBgn0087008 | PBAF complex, chromatin binding and gene silenecing | 0.0596 | . | |||
| nej | FBgn0261617 | Acetylates histone proteins and regulated gene expression | 0.1420 | NS | |||
| E(bx) | FBgn0000541 | Nucleosome remodelling, chromatin organization | 0.0809 | NS | |||
| Pcl | FBgn0003044 | Chromatin binding, co-localizes with the ESC/E(Z) complex | 0.0439 | * | |||
| Interpro/GOTERM_MF_DIRECT | Zinc finger, DNA binding | 4.64 | E(var)3-9 | FBgn0260243 | Chromatin maintenance | 0.0014 | ** |
| Br140 | FBgn0033155 | Enhancer of polycomb-like | 0.0027 | ** | |||
| MTA1-like | FBgn0027951 | Chromatin binding, chromosome condensation | 0.0665 | . | |||
| spn-E | FBgn0003483 | Chromatin binding, chromosome condensation | 0.0153 | * | |||
| hang | FBgn0026575 | Response to oxidative stress | 0.0031 | ** | |||
| phol | FBgn0035997 | Polycomb group protein recruitment to polycomb response elements | 0.0006 | *** | |||
| Chd1 | FBgn0250786 | Remodelling and assembly of chromatin | 0.0865 | . | |||
| Cp190 | FBgn0000283 | Chromatin binding | 0.0165 | * | |||
| cg | FBgn0000289 | Binds to polycomb response elements | 0.0047 | ** | |||
| su(Hw) | FBgn0003567 | Negative regulation of chromatin silencing | 0.0058 | ** | |||
| Chd3 | FBgn0023395 | Chromatin assembly or disassembly, nucleosome remodelling | 0.0027 | ** | |||
| GOTERM_BP/MF/CC_DIRECT, INTERPRO | DNA replication, MCM complex | 4.42 | Orc1 | FBgn0022772 | origin recognition complex, DNA replication, chromatin binding | 0.0013 | ** |
| Orc4 | FBgn0023181 | Initiation of DNA replication | 0.0136 | * | |||
| Orc2 | FBgn0015270 | DNA replication, chromatin binding | 0.0004 | *** | |||
| Mcm5 | FBgn0017577 | Chromatin binding, chromosome condensation | 0.0008 | *** | |||
| RecQ4 | FBgn0040290 | DNA stability, DNA rewinding | 0.0047 | ** | |||
| Mcm10 | FBgn0032929 | Heterochromatin organisation, chromatin silencing | 0.0008 | *** | |||
| polybromo | FBgn0039227 | Chromatin binding | 0.0104 | * | |||
| GOTERM_BP/MF/CC_DIRECT, INTERPRO | Microtublues, kinesin | 4.05 | cid | FBgn0040477 | Histone H3 variant, epigenetic mark for centromere identity | 0.0000 | *** |
| GOTERM_MF_DIRECT, INTERPRO | Helicase | 3.10 | XNP | FBgn0039338 | Heterochromatin organization, chromatin silencing | 0.0924 | . |
| me31B | FBgn0004419 | Gene silencing by miRNA | 0.0001 | *** | |||
| Mi-2 | FBgn0262519 | Chromatin binding, nucleosome binding | 0.0261 | * | |||
| INTERPRO | Structural maintenance of chromosomes | 2.61 | SMC2 | FBgn0027783 | Chromatin binding, chromosome condensation | 0.0020 | ** |
| glu | FBgn0015391 | Chromatin binding, chromosome condensation | 0.0146 | * | |||
| SMC1 | FBgn0040283 | Chromatin binding | 0.0263 | * | |||
| SMC3 | FBgn0015615 | Chromatin binding | 0.0129 | * | |||
| GOTERM_CC/BP_DIRECT | Rb-E2F complex, Myb complex | 2.32 | mor | FBgn0002783 | Brahma associated proteins complex, PBAF complex | 0.0100 | * |
| INTERPRO, GOTERM_MF_DIRECT | Chromo domain | 1.99 | Chro | FBgn0044324 | Histone binding, chromosome organization | 0.0098 | ** |
| Chd1 | FBgn0250786 | Remodelling and assembly of chromatin | 0.0865 | . | |||
| Mi-2 | FBgn0262519 | Chromatin binding, nucleosome binding | 0.0261 | * | |||
| msl-3 | FBgn0002775 | Methylated histone binding, chromatin binding | 0.1760 | NS | |||
| Chd3 | FBgn0023395 | Chromatin assembly or disassembly, nucleosome remodelling | 0.0027 | ** | |||
| GOTERM_MF/BP_DIRECT | Nucleosome mobilisation, nucleosome binding | 1.76 | dre4 | FBgn0002183 | Chromatin binding, nucleosome binding | 0.0021 | ** |
| E(bx) | FBgn0000541 | Histone binding, chromatin organization, chromatin remodelling | 0.0809 | . | |||
| Su(z)12 | FBgn0020887 | Polycomb repressive complex 2, histone methyltransferase activity' | 0.0391 | * | |||
| INTERPRO | WD40 repeat | 1.67 | Hira | FBgn0022786 | Chromatin binding | 0.0000 | *** |
| Caf1-105 | FBgn0033526 | Chromatin assembly factor, histone binding | 0.0001 | *** | |||
| wds | FBgn0040066 | Histone acetyltransferase activity, chromatin remodelling, Trx complex | 0.0032 | ** | |||
| ebi | FBgn0263933 | Chromatin binding | 0.0070 | ** | |||
| GOTERM_BP/CC_DIRECT | Histone phosphorylation | 1.6 | borr | FBgn0032105 | Histone phosphorylation, chromatin binding | 0.0006 | *** |
| ball | FBgn0027889 | Histone threonine kinase activity, histone phosphorylation | 0.0009 | *** | |||
| aurB | FBgn0024227 | Chromatin organization | 0.0004 | *** |
Genes that were significantly up-regulated in these data in HPLC compared to LPHC were compared to a background list of genes that were expressed and detected in these data. Analyses of variance were carried out on expression between all samples in this study (F0-F3 matched and mismatched) with P-values and statistical significances listed.